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      tTMB-P9(matrix) Reference Standard

      CBP80001-9

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      I. Background

      Tumor mutational burden (TMB) shows great promise as a biomarker for immuno-oncology (I-O) therapy response, but progress is hindered by the lack of a standardized TMB measurement by targeted NGS panels, and an absence of TMB reference materials.

       

      Sample ID

      Description

      Cut off=1%

      Cut off=2%

      Cut off=3%

      Cut off=4%

      Cut off=5%

      DC208D0421

      10%T+90%N

      22.94

      19.72

      16.53

      12.50

      8.41

      DC208D0422

      5%T+95%N

      15.75

      10.50

      5.82

      2.92

      1.37

      DC208D0423

      2%T+98%N

      8.29

      2.12

      0.75

      0.42

      0.21

      DC208D0424

      1%T+99%N

      4.15

      0.95

      0.27

      0.18

      0.09

      500xWES;
      IDT xGenExome Research Panel v1.0捕获,Target Region 39M, Probe Region 51M;
      HiSeq X-TEN,af选择0.01 cut off,call突变,再过滤,然后在按照梯度cut off计算TMB。
      Tumor sample: stage 4, adenocarcinoma Lung, Female
      Normal sample: B lymphoblast from the same individual

       

      计算数据下载:

      DC208D0421.filter.0.01.tag

      DC208D0423.filter.0.01.tag

      DC208D0421.filter.0.02.tag

      DC208D0423.filter.0.02.tag

      DC208D0421.filter.0.03.tag

      DC208D0423.filter.0.03.tag

      DC208D0421.filter.0.04.tag

      DC208D0423.filter.0.04.tag

      DC208D0421.filter.0.05.tag

      DC208D0423.filter.0.05.tag

      DC208D0422.filter.0.01.tag

      DC208D0424.filter.0.01.tag

      DC208D0422.filter.0.02.tag

      DC208D0424.filter.0.02.tag

      DC208D0422.filter.0.03.tag

      DC208D0424.filter.0.03.tag

      DC208D0422.filter.0.04.tag

      DC208D0424.filter.0.04.tag

      DC208D0422.filter.0.05.tag

      DC208D0424.filter.0.05.tag

       

       
       
       
       

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